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KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: A conserved PDZ Binding Motif in aPKC interacts with Par-3 and mediates cortical polarity

doi: 10.1016/j.cub.2019.12.055

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Doe Lab N/A D. melanogaster: elav-Gal4, UAS-mCD8:GFP, hs:flp; FRT-G13, tubPGal80 Bloomington Drosophila Stock Center RRID:BDSC_5145 Oligonucleotides Recombinant DNA pCMV mammalian expression plasmid ThermoFisher 10586014 pMal C4X bacterial expression plasmid Addgene 75288 pGex 4Ti bacterial expression plasmid Amersham 27458001 pUASTattB fly cloning and transformation plasmid Addgene EF362409.1 Software and Algorithms ImageJ GraphPad Other PD10 Desalting columns 95017–001 VivaSpin 20 sample concentrators MWCO 30kD VWR 95056–130 VivaSpin 20 sample concentrators MWCO 10kD VWR 95056–128 Shaker Flasks – 125mL VWR 89095–258 Open in a separate window KEY RESOURCES TABLE Par-3 and Par-6/aPKC interact through a PDZ2 – PDZ Binding Motif interaction The Par-3 PDZ2 interaction with the aPKC PBM is conserved across metazoan The aPKC PBM is required for cortical polarity in Drosophila neuroblasts

Techniques: Diagnostic Assay, Recombinant, Construct, Variant Assay, Expressing, Blocking Assay, Plasmid Preparation, Clone Assay, Transformation Assay, Software